| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241338 |
| Chromosome: | chromosome 9 |
| Location: | 5730534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401738 | (1 of 1) K17619 - magnesium-dependent phosphatase 1 [EC:3.1.3.48 3.1.3.-] (MDP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGCAGGCAGCCCTGCTTGCGTACGCG |
| Internal bar code: | AGTGCGGGGTCTGGGGTCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.705 |
| LEAP-Seq n confirming: | 1014 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGAAGTGGTGCTGAC |
| Suggested primer 2: | GTTGACATGGTGGGGGATAG |