Insertion junction: LMJ.RY0402.241338_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241338
Chromosome:chromosome 13
Location:2878221
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGAACGAAGCTGCGCTTCACAACGATGA
Internal bar code:GGCTGGAACGAAACGGGAACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:206
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTCCAGGCAGAAGAAGG
Suggested primer 2:TTGAGCATGGGGTAAGATCC