| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241372 |
| Chromosome: | chromosome 10 |
| Location: | 3189302 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442450 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACACGCGCCCTACTTGCCGCTGCCTA |
| Internal bar code: | GCTAAGTAGGACAAGGTCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 87.2679 |
| LEAP-Seq n confirming: | 987 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCACTCTACCCTCCCGA |
| Suggested primer 2: | GAGAATGGGCTGTCCCAATA |