| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241375 |
| Chromosome: | chromosome 7 |
| Location: | 2523430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329650 | (1 of 1) PTHR23004//PTHR23004:SF7 - DOUBLECORTIN DOMAIN CONTAINING 2 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGAAGATCCTTTGAATAATCAGTGCC |
| Internal bar code: | GGTGGCCCGCGGGTATCTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 99.3624 |
| LEAP-Seq n confirming: | 3117 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTGGGATTGCAGAGTG |
| Suggested primer 2: | TCACATTGAAGCTGTCCTCG |