Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.241375 |
Chromosome: | chromosome_7 |
Location: | 2523430 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g329650 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCTTGAAGATCCTTTGAATAATCAGTGCC |
Internal bar code: | GGTGGCCCGCGGGTATCTGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 192 |
LEAP-Seq percent confirming: | 99.3624 |
LEAP-Seq n confirming: | 3117 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGGTGGGATTGCAGAGTG |
Suggested primer 2: | TCACATTGAAGCTGTCCTCG |