Insertion junction: LMJ.RY0402.241384_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.241384
Chromosome:chromosome 1
Location:1552812
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g008100 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGGGTCCCATCACCCGTTGCAAGTTGT
Internal bar code:CCGAGGCCTTTGGAACGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:481
LEAP-Seq percent confirming:98.6993
LEAP-Seq n confirming:2580
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCCCATGTAACCTGAGT
Suggested primer 2:GAACAGCTCACGCGATGATA