Insertion junction: LMJ.RY0402.241384_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241384
Chromosome:chromosome 1
Location:1552814
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g008100 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGAATGCGTGCGTTTCGACCTTCGGCA
Internal bar code:CGGGAGGAGGGCATAAAAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:99.4932
LEAP-Seq n confirming:589
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCCCATGTAACCTGAGT
Suggested primer 2:GAACAGCTCACGCGATGATA