Insertion junction: LMJ.RY0402.241386_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241386
Chromosome:chromosome 3
Location:5449195
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g185250 SSS2,SSII (1 of 1) IPR001296//IPR011835//IPR013534//IPR015421 - Glycosyl transferase, family 1 // Bacterial/plant glycogen synthase // Starch synthase, catalytic domain // Pyridoxal phosphate-dependent transferase, major region, subdomain 1; Soluble starch synthase II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGGCTCCACGGAGCCGCGCCAGGTCAT
Internal bar code:GAGGCGGCGGCCGAGCTAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:94
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:404
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTGCGTATGCTTTCTGA
Suggested primer 2:CATGTTGTGGATGACGAAGG