| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241443 |
| Chromosome: | chromosome 1 |
| Location: | 3373016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021450 | TPR4 | Tetratricopeptide-repeat protein 4; (1 of 1) PTHR23083//PTHR23083:SF443 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACCACCGTCGCCCCGGCCCTGGCTGTG |
| Internal bar code: | TTGGCATCCGTTCGCCGTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 99.5452 |
| LEAP-Seq n confirming: | 3940 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGATCGTGTGTACCGCA |
| Suggested primer 2: | AGTATTAAGCAAGCGCACGG |