| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241446 |
| Chromosome: | chromosome 13 |
| Location: | 5117234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607450 | DAK1 | Dihydroxyacetone kinase; (1 of 1) 2.7.1.28 - Triokinase / Triose kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGCCATGTCCAGGGAGGTCATGAAG |
| Internal bar code: | TGGGCCTCGCAGTAATTGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.5179 |
| LEAP-Seq n confirming: | 4335 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAAATATGACCCGCACC |
| Suggested primer 2: | CTCCAAGAAGGCAACTCCTG |