Insertion junction: LMJ.RY0402.241451_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241451
Chromosome:chromosome 7
Location:5239807
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g349000 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATACCTACGAGGTGGAGCAAAAGGGCG
Internal bar code:GCGTGTTACCAATTGGTGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:104
LEAP-Seq percent confirming:97.5309
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCGGTGCACAGTCTAT
Suggested primer 2:CCGCTTGTGCCTTCTATCTC