Insertion junction: LMJ.RY0402.241458_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241458
Chromosome:chromosome 3
Location:4890761
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCCAAGCGGGCCGGACCCAGCCATGTC
Internal bar code:GCTGTTTCCGGCGCGGGTGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:303
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACTACAGGGGACAGTGGGG
Suggested primer 2:CGGGCAGGAGTAGAAGAGTG