| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241469 |
| Chromosome: | chromosome 6 |
| Location: | 2104601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265150 | (1 of 1) K11833 - ubiquitin carboxyl-terminal hydrolase 2/21 [EC:3.4.19.12] (USP2_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCAGATCTTCCGCCCGCCGCGCGTGC |
| Internal bar code: | AGCACGCAGTTAGCACAGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 73.2777 |
| LEAP-Seq n confirming: | 1404 |
| LEAP-Seq n nonconfirming: | 512 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTAGAGTGGAGGGGA |
| Suggested primer 2: | CATCCACCCCGATGAAATAC |