| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241517 |
| Chromosome: | chromosome 10 |
| Location: | 484643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421050 | (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTGCATCCGTTCCCAGCCAAAGCCTG |
| Internal bar code: | ACGGCGCGGGGGAGGCAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 98.9899 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTAGGCTGGCTTGATGAG |
| Suggested primer 2: | CCACCACACCGTACACAGAC |