| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241519 |
| Chromosome: | chromosome 3 |
| Location: | 2130506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157100 | (1 of 1) PF03420 - Prohead core protein serine protease (Peptidase_S77) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTCAATGTCGCTGCGACTCAACGTCTG |
| Internal bar code: | AGCTCGGGTACGACAATGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 99.4869 |
| LEAP-Seq n confirming: | 1939 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCAATGGAGAAAGTGT |
| Suggested primer 2: | GACTACAGCCATGCTGCTCA |