| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241536 |
| Chromosome: | chromosome 12 |
| Location: | 5228899 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528150 | OST3 | (1 of 2) PF04756 - OST3 / OST6 family (OST3_OST6); Oligosaccharyl transferase complex subunit 3 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAGGGGCGCTGCAGTTTTCCGAAGCA |
| Internal bar code: | CGCAATGTGCGTCTGAATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 85.489 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGCGGTGGCTTCTTCTA |
| Suggested primer 2: | GACACACTCCACCTTCGTGA |