| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241540 |
| Chromosome: | chromosome 9 |
| Location: | 2791717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389000 | (1 of 1) PTHR23012:SF61 - AT07234P-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTCGGCGAGCATTCTTGAACGGGCCAA |
| Internal bar code: | CCGCAAATGCGAAGCCACCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 99.1745 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTATTGTACCAGGCGGC |
| Suggested primer 2: | GCTAATCTACCAAACGGCCA |