| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241542 |
| Chromosome: | chromosome 17 |
| Location: | 1535815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707300 | PGA3 | Phospholipid/glycerol acyltransferase; (1 of 1) PTHR23063:SF1 - 1-ACYLGLYCEROPHOSPHOCHOLINE O-ACYLTRANSFERASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACAGAGCCGCGACAACCACCGCGCCC |
| Internal bar code: | CGTCCAATCCTGATGGGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 98.0668 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAAATCCCTGTTGCTAA |
| Suggested primer 2: | GCACCACAACCAACAACAAG |