Insertion junction: LMJ.RY0402.241548_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241548
Chromosome:chromosome 7
Location:3645564
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g337125 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGACTGCCGCCGCTGCGCGTGGCACCCG
Internal bar code:CCTCGTTAGCCCAGACAGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:94.5055
LEAP-Seq n confirming:430
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCTTGTTTGCGGTTTCT
Suggested primer 2:GTCCTTTGCGAAGCTTGTTC