Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.241548 |
Chromosome: | chromosome 7 |
Location: | 3645564 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g337125 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGACTGCCGCCGCTGCGCGTGGCACCCG |
Internal bar code: | CCTCGTTAGCCCAGACAGGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 587 |
LEAP-Seq percent confirming: | 94.5055 |
LEAP-Seq n confirming: | 430 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATCCTTGTTTGCGGTTTCT |
Suggested primer 2: | GTCCTTTGCGAAGCTTGTTC |