| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241571 |
| Chromosome: | chromosome 3 |
| Location: | 4341826 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175050 | TRP13 | Transient receptor potential ion channel protein; (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCCGCGCGGATGCCGGTAGGTCTTGGA |
| Internal bar code: | GAGTCGCTAGTGGCATTCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 99.6622 |
| LEAP-Seq n confirming: | 590 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTATGCAGCTGTTCATCA |
| Suggested primer 2: | AACCGTGACTGACCTTCACC |