| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241585 |
| Chromosome: | chromosome 10 |
| Location: | 733153 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422718 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGAGCCCCGGAACTTCATGCAACCCCC |
| Internal bar code: | GCTATGGCTCGGGCGTGCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 91.9492 |
| LEAP-Seq n confirming: | 217 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTAAGAGCGCACCTTT |
| Suggested primer 2: | GGTAGAAATTTCGGTGGCAA |