| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241592 |
| Chromosome: | chromosome 1 |
| Location: | 7110263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051400 | ERD2A | KDEL Receptor A | 5'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGCACCAGACGAGGCAAGCCTCTTTT |
| Internal bar code: | GCCGTTGAGCCGCCAATAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.6942 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAAAGGTCCACAGCAC |
| Suggested primer 2: | CTACGCCGCATATTGGAAAT |