| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241637 |
| Chromosome: | chromosome 1 |
| Location: | 3661405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023600 | (1 of 8) PTHR10366:SF411 - HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTATAGTACAGTTGATCACAAGTAAC |
| Internal bar code: | CTATATTCTTACGGCGTTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 90.046 |
| LEAP-Seq n confirming: | 1565 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTCACAGACCTGGTGTA |
| Suggested primer 2: | TCGGCTGCTAGATAGCTGGT |