Insertion junction: LMJ.RY0402.241711_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241711
Chromosome:chromosome 17
Location:4123015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729600 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCATCAACTACCGCCAACCACCAGCCAA
Internal bar code:GTAGGGGAACACGAAGGATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:90.6606
LEAP-Seq n confirming:398
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATCCAATCCCAAACCAT
Suggested primer 2:CTAGTAATGTGGCGACGGGT