| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241716 |
| Chromosome: | chromosome 17 |
| Location: | 2405734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715350 | (1 of 1) K12493 - ADP-ribosylation factor GTPase-activating protein 2/3 (ARFGAP2_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCAGTTCGCCCGCTGTGCATGGTGCGA |
| Internal bar code: | CAACATTGGGGGGCTTCGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.3403 |
| LEAP-Seq n confirming: | 4066 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCATACAGCACTCCCA |
| Suggested primer 2: | AAGAGTCTTACAAGCGGGCA |