| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241728 |
| Chromosome: | chromosome 4 |
| Location: | 701189 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217954 | (1 of 1) K05850 - Ca2+ transporting ATPase, plasma membrane (ATP2B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACACACGCATCGACACGCACTCCCATC |
| Internal bar code: | AGCTTGGGATTCGGAGCTCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.4426 |
| LEAP-Seq n confirming: | 892 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGATATCGGATTGGAGG |
| Suggested primer 2: | AGCGGTCACGGTCTAAGAGA |