Insertion junction: LMJ.RY0402.241734_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.241734
Chromosome:chromosome 1
Location:4803056
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g033200 (1 of 3) IPR001510 - Zinc finger, PARP-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTCCGACCATGGTCTCCGGGGTCGGCT
Internal bar code:AATGCCATGGTGACCAAGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:99.0654
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGCACTGTAAAGGAGCC
Suggested primer 2:CGCCAGACTGGTTCTCTAGG