| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241743 |
| Chromosome: | chromosome 2 |
| Location: | 4836561 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103100 | SDR5 | (1 of 1) K11165 - dehydrogenase/reductase SDR family member 7 (DHRS7); Short-chain dehydrogenase/reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCTGTGGGAGACACGCTCGGTCTGCC |
| Internal bar code: | GCGTCCTGTGGTTTTGTTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.3516 |
| LEAP-Seq n confirming: | 19920 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCGCAGCATCACCTTCT |
| Suggested primer 2: | TAAAGCAAGGCCCTGTCACT |