| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241749 |
| Chromosome: | chromosome 7 |
| Location: | 3263369 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334300 | TRP6 | Transient receptor potential ion channel protein; (1 of 2) PTHR22847//PTHR22847:SF442 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGGCTGGGTTCTGCAAGACATGGAGC |
| Internal bar code: | TGCTGTCCGTGTTCTGGTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 99.0033 |
| LEAP-Seq n confirming: | 298 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGTCCTCTCCTTGAGC |
| Suggested primer 2: | GCACTCCCATCCCTACTCAA |