| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241768 |
| Chromosome: | chromosome 12 |
| Location: | 5240488 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACATAACTTGGCTTCCTGGCTAGTAGA |
| Internal bar code: | CGCCCCCGGCCAGTAAAGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 98.8258 |
| LEAP-Seq n confirming: | 4124 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGAGTACGTCGGTGGAC |
| Suggested primer 2: | GTTGCTGAGTGGTGAAGCAA |