| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241788 |
| Chromosome: | chromosome 4 |
| Location: | 342531 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217909 | OPR124 | OctotricoPeptide Repeat protein 124 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAACCAACCCATTTCGCACCGCTCTGT |
| Internal bar code: | CGGACGGATGTCGAGCAAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 97.7612 |
| LEAP-Seq n confirming: | 262 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTACTCTTGGCGCTGC |
| Suggested primer 2: | AGAACCTAGGGTGTGGGCTT |