Insertion junction: LMJ.RY0402.241791_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241791
Chromosome:chromosome 12
Location:6810872
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g561550 CDS1 Mitochondrial half-size ABC transporter, membrane protein; (1 of 2) K05661 - ATP-binding cassette, subfamily B (MDR/TAP), member 6 (ABCB6) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACTGAGCAAACCCTCTCATGACCATAC
Internal bar code:TCAACATCGGGCCCGTACACC

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:99.8043
LEAP-Seq n confirming:510
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGTTGACTCTGATGCC
Suggested primer 2:GACATGACATGGTCTCGTGG