Insertion junction: LMJ.RY0402.241822_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.241822
Chromosome:chromosome 3
Location:7970965
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAGTCCTCCAACTCGTACCAGACCACA
Internal bar code:GAACCGAGGAGAGGATTGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:89.8438
LEAP-Seq n confirming:115
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCTTCTTCTTCTTGGGC
Suggested primer 2:CAGGTCACAACATGGGTCTG