| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241839 |
| Chromosome: | chromosome 16 |
| Location: | 1868080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655600 | (1 of 4) PF16016 - Domain of unknown function (DUF4782) (DUF4782) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACTGGGGTGTTGAGCAGAGTGTCAC |
| Internal bar code: | TTTTGCTGTCGTCACCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 99.759 |
| LEAP-Seq n confirming: | 1242 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACGGGTATCCTGCTGT |
| Suggested primer 2: | CCAGTCCATGCCAACTACCT |