| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241864 |
| Chromosome: | chromosome 12 |
| Location: | 6018993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535050 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGCTGCCGCCGTCCCTGTCTGAGGAAC |
| Internal bar code: | ATTAGATTTTGAACCATTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 98.234 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCAGTCAACTGTCGT |
| Suggested primer 2: | ACGCCTTATCAACACGTTCC |