| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.241904 |
| Chromosome: | chromosome 3 |
| Location: | 8252989 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTGCCCAGTAGTTGGCAGTATGCAGG |
| Internal bar code: | CAAGCTCTTCGGGACCAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 98.6395 |
| LEAP-Seq n confirming: | 290 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTTCAGGCGTGAGAACA |
| Suggested primer 2: | GATCCAGCGTCATGGTGTC |