| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241904 |
| Chromosome: | chromosome 14 |
| Location: | 3568558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631100 | CEP13,SRR29 | Cysteine endopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTAATGGCACCTGGTACTGGGATGAC |
| Internal bar code: | CCGCAACCTTAGGAACGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.7308 |
| LEAP-Seq n confirming: | 741 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTACAGGAAGGATTGCC |
| Suggested primer 2: | TTGCTGTCATTCGTAGGCTG |