Insertion junction: LMJ.RY0402.241910_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.241910
Chromosome:chromosome 3
Location:4297704
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174672 (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTGTTGATGCCATCAACCAGGGTGCGG
Internal bar code:GCTGCCAATCCAGCATAGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTTTCACTCACCCCCT
Suggested primer 2:ACACTCGTCATACCATGCCA