| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241911 |
| Chromosome: | chromosome 14 |
| Location: | 3143676 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628850 | CYG26 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTAGCCGCGCGCACGCACGGAATGACGC |
| Internal bar code: | CAGACTCTTTAAAGAAATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 99.3548 |
| LEAP-Seq n confirming: | 462 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCACCAGTAAGGAGCAG |
| Suggested primer 2: | TTGTCGCATGTCCAAAATGT |