| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.241917 |
| Chromosome: | chromosome_4 |
| Location: | 3486881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g227900 | PF20 | WD-repeat protein of flagellum central pair | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCGGTATGTGTGTTAGCAAAGCACAATGG |
| Internal bar code: | TGGTTGTACGAGCTTTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.5527 |
| LEAP-Seq n confirming: | 2448 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGGAGAGAGAGCAGTT |
| Suggested primer 2: | ACTCGAGTTTGGAAGAGCCA |