Insertion junction: LMJ.RY0402.241926_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241926
Chromosome:chromosome 11
Location:2482010
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGACCTGCCGTGCGTGCTCCACTCGCC
Internal bar code:TTCCAAGATAGTTGCGGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:96
LEAP-Seq percent confirming:96.2617
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCAATGTGTCTGTGCGACAA
Suggested primer 2:CACGTTCAACCAATACACGC