| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241930 |
| Chromosome: | chromosome 9 |
| Location: | 180246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386740 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTTTACACTCAGGGCGCCGATAAATGC |
| Internal bar code: | TATGGGATTCCGCCAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 81.0811 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCACCCAACCTACCATC |
| Suggested primer 2: | CTAGGCCTTGGCTGTCTCAC |