Insertion junction: LMJ.RY0402.241942_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.241942
Chromosome:chromosome 7
Location:5092483
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g347700 (1 of 1) PF10220 - Uncharacterized conserved protein (DUF2146) (DUF2146) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTTCCTACACCCTCTGAACAACGGCCGA
Internal bar code:TATTCCGCTTAACAAGAGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:134
LEAP-Seq percent confirming:23.5955
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:136
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCAACTTGGAAACAAGC
Suggested primer 2:CTGCAACAGGTAGCAAGCC