| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241944 |
| Chromosome: | chromosome 14 |
| Location: | 961576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613900 | CYCT1 | (1 of 2) PTHR10026//PTHR10026:SF51 - CYCLIN // CYCLIN-K; T-type cyclin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCCTGCAGGCTGATGCAGAGCTGTGT |
| Internal bar code: | GCATGCGGGTACTAGGCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 86.2069 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCTAGAACTCCACAGG |
| Suggested primer 2: | GTCAGCCCAAATTAAACCGA |