| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.241968 |
| Chromosome: | chromosome 1 |
| Location: | 3065952 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018950 | DHS1 | Deoxyhypusine synthase; (1 of 2) 2.5.1.46 - Deoxyhypusine synthase / Spermidine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCACGTCAGCGCCCATTTTGCGCCAG |
| Internal bar code: | AGGGGGCGCAAATTGCCAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 270 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCTCCACCAGATAGCC |
| Suggested primer 2: | TGAGTGTTGGGTTTTGTGGA |