| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242018 |
| Chromosome: | chromosome 1 |
| Location: | 3157069 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACTAGGGTGCAGGCCAAGGGCCAAAGC |
| Internal bar code: | AAATGGAGAGTACAGATCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 98.8796 |
| LEAP-Seq n confirming: | 1059 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTGATGATTGGGGTTC |
| Suggested primer 2: | GCTTTCGTAACACGCACAAA |