| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242041 |
| Chromosome: | chromosome 2 |
| Location: | 4631555 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101150 | (1 of 2) IPR000104//IPR000210//IPR011333 - Antifreeze protein, type I // BTB/POZ domain // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCGAGTGGCGTGGCGTGGGCGTTGCC |
| Internal bar code: | GGTGATATATGTTCTTGTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 98.3452 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAGCCGTACCTGTGAC |
| Suggested primer 2: | GTGAGTCGTGGGTTGGTCTT |