Insertion junction: LMJ.RY0402.242041_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.242041
Chromosome:chromosome 9
Location:2158465
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g393300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGGGGGACGGGGTGCTGGGGGGCATGG
Internal bar code:GCTACCCGGCAAATGTGCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1104
LEAP-Seq percent confirming:92.8499
LEAP-Seq n confirming:16453
LEAP-Seq n nonconfirming:1267
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACAGCGTCAAG
Suggested primer 2:ACACACACACACACACACGC