Insertion junction: LMJ.RY0402.242041_5

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242041
Chromosome:chromosome 9
Location:2158526
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g393300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCTCAGGCGTTTTCCACCCGGATGATCA
Internal bar code:TATTGCTACGAGGCGCAGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:99.6183
LEAP-Seq n confirming:261
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACAGCGTCAAG
Suggested primer 2:ACACACACACACACACACGC