| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242050 |
| Chromosome: | chromosome 3 |
| Location: | 3410708 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166950 | PGM5 | (1 of 1) K01834 - 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM, gpmA); Phosphoglycerate mutase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTGGCTTGTTGCCAAATTAAACCAGG |
| Internal bar code: | TAAACAACGGTCCATGCCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 99.3197 |
| LEAP-Seq n confirming: | 438 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGACCCGAGGACAGTTC |
| Suggested primer 2: | GATGACGGTAGGTGCTGGTT |