| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242056 |
| Chromosome: | chromosome 3 |
| Location: | 5871714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189650 | (1 of 1) K04498 - E1A/CREB-binding protein (EP300, CREBBP, KAT3); Histone acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACCGCGGGGCCCCATGACGTTTCACTT |
| Internal bar code: | GGAATTCAGTTTTCACTCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 228 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCGGACAAATTAGTGG |
| Suggested primer 2: | GGATTGATGGTGCTCTGGTT |